Greetings,

I have a future project of interest. I have been examining trace files of DNA sequencing. These consist of four colored plots of peaks. I am very close to being able to abstract the data for a region of interest (approximately 20 bases) from the *.ab1 files and plot it. These would be troublesome reads that do not lend themselves to an automated call. What I envision might be a place to display data *and* collect the call, which would be of length 2 at most and restrict it to A, C, G, T, N, combinations thereof, or missing. I would use SAS to assemble my data and display it (i.e. a data step and a PLOT procedure). To what might I write it? For instance, to HTML? Might I consider SCL? I might be more enticed by a "display" that could be independent of SAS, but I also conceive several advantages of write the call directly to a transistion data set or directly to my data set of interest. I appreciate any comments.

Thanks,

Kevin

Kevin Viel Department of Epidemiology Rollins School of Public Health Emory University Atlanta, GA 30322